课题组成员：

教授: 李程

研究生：王国良，金梦怡，康红花，高茹心，郑岚，范凯，林经纬，杨秀琴，叶颖钥，王梦媛，刘泽宇，陈翠婷

Tel: 0592-2183761

E-mail: cheng-li@xmu.edu.cn

主要研究领域：

角膜和眼表疾病的发生机理及调控机制

角膜上皮干细胞，作为角膜上皮的唯一细胞来源，对维持角膜上皮的动态稳定和角膜透明性发挥着极其重要的作用。角膜上皮干细胞功能障碍(limbal stem cell dysfunction，LSCD)的患者由于干细胞数量的缺乏或定向分化潜能丢失，往往表现为眼表组织广泛破坏，发生持续性角膜上皮缺损、慢性炎症、角膜上皮结膜化和新生血管侵入，继而角膜透明度下降，造成患者严重视力障碍甚至失明。大量研究证明，p38MAPK, Wnt, Notch等多种信号传导通路参与并调节了LSCD的发生进程。深入研究多条通路之间交叉作用的具体机制，必将为我们认识角膜上皮干细胞的生物学特性，阐明LSCD的发病机理提供重要信息。

角膜上皮干细胞增殖分化的相关信号通路以及LSCD发生的可能机制

代表性论文：

1.Han Y#, Zheng L#, Wang Y, Fan K, Guo S, Kang H, Lin J, Xue Y*, Liu Z*, Li C*. Corneal stromal filler injection of gelatin-based photocurable hydrogels for maintaining the corneal thickness and reconstruction of corneal stroma. Composites Part B, 2023, Nov 266; 111004

2.Wang G#, Xu L#, Shi R, Ye Y, Zeng B, Yang X, Liu Z, Liu Z, Wang S*, Xue Y*, Li C*. Organotypic culture model of mouse meibomian gland as a screening platform for risk factors related to meibomian gland dysfunction. Ocul Surf. 2023 Aug 22; 30:73-84.

3.Xu L#, Wang G#, Shi R, Zeng B, Zhang Y, Liu Z, Wang S*, Li C*. A Cocktail of Small Molecules Maintains the Stemness and Differentiation Potential of Conjunctival Epithelial Cells. Ocul Surf, 2023 Aug 25;30:107-118.

4.Han Y#, Guo S#, Li Y#, Li J, Zhu L, Liu Y, Lv Y, Yu D, Zheng L, Huang C, Li C*, Hu J*, Liu Z*. Berberine ameliorate inflammation and apoptosis via modulating PI3K/AKT/NFκB and MAPK pathway on dry eye. Phytomedicine. 2023 Sep, 155081.

5.Yang D, Han Y, Wang Y, Pan Y, Zheng L, Liu Z*, Li C*, Wu Y*, Li H*. Highly effective corneal permeability of reactive oxygen species-responsive nano-formulation encapsulated cyclosporine a for dry eye management. Chem Eng J. 2023; 469: 143968

6.Kang H, Han Y, Jin M, Zheng L, Liu Z, Xue Y*, Liu Z*, Li C*. Decellularized squid mantle scaffolds as tissue-engineered corneal stroma for promoting corneal regeneration. Bioeng Transl Med. 2023; e10531

7.Xu L, Hou S, Huang X, Wang M, Li C*, Dong N*, Lin Z*. Highly sensitive homogeneous electrochemiluminescence biosensor for microRNA-155 based on enzyme-free cascade signal amplification and magnetic assisted enrichment. J Elec Chem. 2023, 933: 117274

8.Tang Y#, Han Y#, Zhao J, Lv Y, Fan C, Zheng L, Zhang Z, Liu Z*, Li C*, Lin Y*. A Rational Design of a Metal-Organic Framework Nanozyme with High-Performance Copper Active Centers for Alleviating Chemical Corneal Burns. Nanomicro Lett. 2023 Apr 30;15(1):112.

9.Bao X, Zhong Y, Yang C, Chen Y, Han Y, Lin X, Huang C, Wang K*, Liu Z*, Li C*. T-Cell Repertoire Analysis in the Conjunctiva of Murine Dry Eye Model. Invest Ophthalmol Vis Sci. 2023 Mar 1;64(3):14.

10.Wang Y#, Kang H#, Jin M, Wang G, Ma W, Liu Z, Xue Y*, Li C*. Phenotypic and Transcriptomics Analyses Reveal Underlying Mechanisms in a Mouse Model of Corneal Bee Sting. Toxins (Basel). 2022 Jul 8;14(7):468.

11.Zhong Y#, Fang X#, Wang X, Lin Y, Wu H*, Li C*. Effects of Sodium Hyaluronate Eye Drops With or Without Preservatives on Ocular Surface Bacterial Microbiota. Front Med. 2022 Feb 14; 9: 793565.

12.Zong R, Zhu F, Han W, Wang Y, Wang G, Wang Y, Mao Y, Guan T, Liu Z, Xue Y*, Li C*. Tear dynamics testing and quantitative proteomics analysis in patients with chronic renal failure. J Proteomics. 2021 Aug 16:104351.

13.Wang G，An X，Jin M, Wang X, Zong R, Liu Z, Liu Z, Xue Y*, Li C*. Real-time confocal microscopy imaging of corneal cytoarchitectural changes induced by different stresses. Exp Eye Res. 2021 Sep; 210: 108706.

14.Li Z, Liu M, Ke J, Wang J, Wu C, Li C*, Li Z*, Wu Y*. Flexible polymeric nanosized micelles for ophthalmic drug delivery: research progress in the last three years. Nanoscale Adv. 2021, 3 (18), 5240-5254

15.Han Y#, Jiang L#, Shi H#, Xu C, Liu M, Li Q, Zheng L, Chi H, Wang Y, Liu Z, You M, Loh X, Wu Y*, Li Z*, Li C*. Effectiveness of an ocular adhesive polyhedral oligomeric silsesquioxane hybrid thermo-responsive FK506 hydrogel in a murine model of dry eye. Bioact Mater. 2021 Jul 28; 9:77-91.

16.Jin M, Wang Y, An X, Kang H, Wang Y, Wang G, Gao Y, Wu S, Reinach P, Liu Z, Xue Y, Li C*. Phenotypic and transcriptomic changes in the corneal epithelium following exposure to cigarette smoke. Environ Pollut. 2021 Oct 15; 287: 117540.

17.An X, Wang G, Jin M, ZhouX, Gao S, Chen J, Reinach P, Liu Z, Xue Y*, Li C*. Novel Cell Culture Paradigm Prolongs Mouse Corneal Epithelial Cell Proliferative Activity in vitro and in vivo. Front Cell Dev Biol. 2021 Jun 30; 9:675998.

18.Han Y#, Xu C#, Shi H#, Yu F, Zhong Y, Liu Z, Loh X, Wu Y*, Li Z*, Li C*. Engineered bio-adhesive polyhedral oligomeric silsesquioxane hybrid nanoformulation of amphotericin B for prolonged therapy of fungal keratitis. Chem Eng J. 2021, 421, 129734.

19.Dong N#*, Cai Q#, Li Z, Xu L, Wu H, Lin Z, Qiu B, Li C*, Lin Z*. Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout. Analyst. 2021 May 17;146(10):3299-3304.

20.Tao Y, Luo F, Lin Y, Dong N*, Li C*, Lin Z*. Quantitative gold nanorods based photothermal biosensor for glucose using a thermometer as readout. Talanta. 2021 Aug 1; 230: 122364.

21.Ma W, Wang G, Li X, Wu H, Liu Z, Dong N*, Li C*. Study of Factors Influencing Dry Eye in Rheumatoid Arthritis. J Ophthalmol. 2020 Aug 8; 2020: 5768679.

22.Wang G, Chen P, Wang Y, Wang Y, Reinach PS, Xue Y, Liu Z*, Li C*. Onion Epithelial Membrane Scaffolds Transfer Corneal Epithelial Layers in Reconstruction Surgery. Adv Healthc Mater. 2020 Jul;9(14): e2000469.

23.Jin M, Wang Y, Wang Y, Li Y, Wang G, Liu X, Xue Y*, Liu Z, Li C*. Protective effects on corneal endothelium during intracameral irrigation using N-(2)-L-alanyl-L-glutamine. Front Pharmacol. 2020 Mar 27; 11: 369.

24.Han Y#, Li C#*, Cai Q, Bao X, Tang L, Ao H, Liu J, Jin M, Zhou Y, Wan Y, Liu Z*. Studies on bacterial cellulose/poly vinyl alcohol (BC/PVA) hydrogel composites as tissue-engineered corneal stroma. Biomed Mater. 2020 Apr 30;15(3):035022.

25.Wang G, Xue Y, Wang Y, Dong F, Shen M, Zong R, Liu Z*, Li C*. The role of autophagy in the pathogenesis of exposure keratitis. J Cell Mol Med. 2019 Jun;23(6):4217-4228.

26.Yang Q, Tang L, Shen M, Wang Y, Wei Y, Jeyalatha V, Chen P, Dong F, Wang G, Wu S, Liu Z*, Li C*. Effects of diesel exhaust particles on the condition of mouse ocular surface. Ecotoxicol Environ Saf. 2018 Nov 15; 163: 585-593.

27.Wang G, Dong N, Wang Y, Li J, Dong F, Jeyalatha V, Wu J, Shen M, Yang Q, Chen P, Xue Y, Liu Z, Li C*. Epithelial dysplasia in pterygium postoperative granuloma. Exp Eye Res. 2018 Oct; 175: 199-206.

28.Li J#, Li C#, Wang G, Liu Z, Chen P, Yang Q, Dong N, Wu H, Liu Z*, Li W*. APR-246/PRIMA-1Met Inhibits and Reverses Squamous Metaplasia in Human Conjunctival Epithelium. Invest Ophthalmol Vis Sci. 2016 Feb;57(2):444-52.

29.Li C, Dong N, Wu H, Dong F, Xu Y, Du H, He H, Liu Z*, Li W*. A novel method for preservation of human corneal limbal tissue. Invest Ophthalmol Vis Sci. 2013 Jun 10;54(6):4041-7.

30.Li C, Dong F, Jia Y, Du H, Dong N, Xu Y, Wang S, Wu H, Liu Z*, Li W*. Notch signal regulates corneal endothelial-to-mesenchymal transition. Am J Pathol. 2013 Sep;183(3):786-95.

31.Li C, Yin T, Dong N, Dong F, Fang X, Qu YL, Tan Y, Wu H, Liu Z*, Li W*. Oxygen tension affects terminal differentiation of corneal limbal epithelial cells. J Cell Physiol. 2011 Sep;226(9):2429-37.